Separation of steroids 5b-pregnane-3a,20a-diol 17b-estradiol and estrone by TLC
Summary
Type: TLC Application #: 401520
Detection: spray with 1% vanillin in conc. sulphuric acid (see chapter visualization reagents) and heat 5 min to 105 °C Conditions: Eluent: ethyl acetate / cyclohexane (1:1, v/v) Migration distance: 10 cm in 20 min, ascending, chamber saturation Authors: TLC department Source: TLC department